• DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. The values of luminescence are directly proportional to the number of viable cells. In addition, the FilterMax F5 Reader is capable of measuring nucleic acids directly at 260 nm in absorbance detection mode. Our newest multimode microplate reader combines simplicity with top-of-the-line performance. Firefly and Renilla luciferase relative light units were measured using the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of performing absorbance, luminescence, and fluorescence intensity measurements on microplate samples (Figure 1-1). Categories:. CloneMatrix. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seEye lysates were developed using a Glucose Colorimetric Assay Kit (Sigma, Louis, MO, USA) and measured by a FilterMax™ F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 570 nm. Bioprocess Equipment and Technology. ScanLater Western Blot Detection System. 40266 Email: [email protected] or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. 25. Many absorbance assays use a chromogenic substrate, which, upon enzymatic conversion to the final product, results in a compound thatThen 200 µl of 10% (v/v) ammonium hydroxide was added to neutralize the acid and absorbance readings were taken at 405 nm with the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices. 00. 3 Pages. ** Some compatible filter sets are available for purchase from Life. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. ScanLater Western Blot Detection System. Topic. Create an experiment based on the protocol. SpectraMax Glo Steady-Luc Reporter Assay Kit. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. 00. BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Time-resolved Fluorescence. Absorbance was detected from the bottom using the FilterMax F5 multimode microplate reader (Molecular Devices, Sunnyvale, CA) at excitation/emission wavelengths of 485 nm/535 nm. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $3,500. Perkin Elmer Wallac Victor2 1420 Multilabel. 3 Pages. 4 Pages. The transmittance at 450 nm was determined by FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA). Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices Supplier: Molecular Devices. Page 25: FeaturesThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seIMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. FilterMax F5 Multi-Mode Microplate Reader. Seoul, South Korea. Catalog. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Aspirate 150 µL from wells C5-F5 and discard. 3 Pages. - September 14, 2010 - Molecular Devices, Inc. A) CHO colonies, serum-free suspension-adapted, in CloneMedia-CHO. J. SpectraMax iD5. 40266 Email: drugdiscoverytech@lifetech. LVF Monochromator™ : Excitation and Emission. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 3 Pages. 561. 00. 3 Pages. Statistical analysis was performed using. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available)Find used microplate readers on Machinio to perform a wide range of assays including ELISAs, cell viability assays, nucleic acid quantification, and more. Each kit was used on the same day that its reagents were reconstituted. performs within its specifications. book Page 1 Tuesday, September 7, 2010 6:14 PM. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Watson. The 0. The FlexStation 3 Benchtop Multi-Mode Microplate Reader can measure absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-The next day, precultures were diluted to an optical density of 0. Upgradeable modules are also available including western blot, cell imaging, and fast. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 2 μM ATP standard was. time plots at 30, 60, and 90 min using MATLAB® R2017b (9. Bio-Tek Microplate Reader Synergy HT $8,995. Immunoblot analysis was performed with pooled serum (diluted 1:500) separated by SDS-PAGE under non-reducing conditions and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). 3 Pages. The ucs load command creates a backup of. The drawing shows a standard microplate of 15 mm height covered with a lid. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. The OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. SpectraMax iD5. Datapaq Express (Vial Rack Bottom Label 2D Reader) XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING. Molecular Devices technical support is available by phone and e-mail. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Description: The FilterMax™ F3 features absorbance (visible),. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. . Not for use in diagnostic procedures. Caspase 3/7 activity was measured according to previously established methods [11,12,13,15,16]. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 4. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). Click Run. FilterMax F5 Multi–Mode microplate reader from Molecular Devices Multi–Mode Analysis software. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. 11 mg/mL in PBS, 20 h incubation) using the FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices (Sunnyvale, CA, USA), at 570 nm . MetaFluor Software. A Vision for the Future With TTP LabTech. FilterMax F5 Multi-mode microplate reader. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Page: 1/4NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. 7°C and the bottom to 36. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. Match case Limit results 1 per page. 4 Pages. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceF3/F5 Multi-Mode Microplate Readers. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. The absorbance of the samples was determined using a FilterMax F3&F5 Multi-Mode Microplate Reader (Molecular Devices). Molecular Devices technical support. Please click on the links below to view the Microplate Reader Comparison Tables. *** (p < 0. 012 fmol 0. 3 Pages. Devices FilterMax Multi-Mode Microplate Readers. Page 4 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 5. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. 8 Pages. SpectraMax iD5. DHE fluorescence was recorded on a Filtermax F5 multi-mode microplate reader. 7°C (gradient heating design). Briefly, serial dilutions of TNP were incubated with 200 TCID 50 virus in presence of diethylaminoethyl-dextran and the neutralizing activity was assessed by measuring luciferase activity after 48 h using a FilterMax F5 multi-mode microplate reader (Molecular Devices). Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. The experiment was performed in 4 technical replicates (wells in the cell culture plate). ** Some compatible filter sets are available for purchase from Life. Both the FilterMax F3 and F5 Readers are capable of detecting nucleic acid concentration by fluorogenic means. 2. 2. 3 and higher include optimized reader settings, as well as data analysis, for faster results. 2. These assays span over a wide wavelength range, generally from 220-900 nm in different detection modes (typically absorbance, fluorescence and luminescence). They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). Supernatant (25 μL), or standard, was added and mixed. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solu. 8 Pages. CloneMatrix. Related Products: FilterMax F3 & F5 Multi-Mode Microplate Readers, SpectraMax i3 Multi-Mode Platform Microplate Detection System,. The quantity of the colorimetric product was monitored continuously for 120 min. Read Review (1)With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Chemiluminescence emission was measured in real time (every 2 min for 120 min) with a FilterMax F5 Multi-Mode Microplate Reader at 27°C and expressed as area under the curve, as previously described . Renilla/Firefly luciferase activities were measured twice. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. ScanLater Western Blot Detection System. The OD 595 of the culture in the plate (light path length: ∼6. To do so, perform one of the. , Sunnyvale, CA, USA). The fluorescence was determined using a fluorimetric microplate reader (FilterMax F5 Multi-Mode Microplate Reader) at 540 and 595 nm for excitation and emission, respectively. Application:. 1 MB Table of Contents. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Fluorescence intensity was determined using a FilterMax F5 multi-mode microplate reader at an excitation wavelength of 485 and an emission wavelength of 530nm. The Hidex Sense is a compact, state-of-the-art, multi-mode microplate reader which provides a powerful and flexible platform for performing any kind of assay without limitations. The values of luminescence are directly proportional to the number of viable cells. The absorbance was measured at 450 nm using a FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp. Multi-Mode Readers. 6 Pages. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. The following document is. 561. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se The method is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) under conditions of high temperature and acidity that generates a fluorochrome, which is quantified by fluorimetry (F5 Multi-Mode Microplate Reader), with excitation at 515 nm and emission at 553 nm. They provide absorbance, fluorescence, glow. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched. 4. ~ Bio-Tek ELx808 Microplate Reader $2,795. i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. After 2–4 h incubation at 37 °C, the medium was removed and 100 μl dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan. 3. Unsuccessful migration. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader with high sensitivity. A 1:1 binding ratio Hill model was used to fit the binding interaction data. This microplate reader also features BioTek’s Hybrid Technology, which. F3/F5 Multi-Mode Microplate ReadersCell pellets were lysed and HTLV-1 LTR-transactivation was measured via luciferase assay according to the manufacturer’s protocol (Dual-Luciferase ® Reporter Assay System, Promega Corporation, Madison, WI; Filtermax F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) . Optical System: High energy xenon flash lamp. Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. 4) onto the FilterMax F5 to be read with SpectraMax® Paradigm® Multi-Mode Detection Platform. Internal controls were. The hemolysis was calculated with the following formula:Luminescence from the converted ATP was then measured using a FilterMax F5 Multi-mode microplate reader (Molecular Devices). Intended Use Statement The Synergy H1 is a hybrid multi-mode microplate reader. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. book Page 1 Tuesday, September 7, 2010 6:14 PM. shopping cart 0 items $0. Add. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Click to Contact Seller. The microplates were incubated at 37°C for 24 h, and the growth of bacteria was determined by measuring the optical density (OD) at 630 nm using an ELISA microplate reader (FilterMax F5 multi-mode microplate reader, Molecular Devices, Sunnyvale, CA, USA). FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#:. Manufacturer: Molecular Devices. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 2. Before unpacking and setting up the FilterMaxTM F3 or FilterMaxTM F5 Microplate Reader, prepare a dry, flat work area that has sufficient space for the instrument, host computer,. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Buffer without protease was used as a blank for background subtraction. 9). FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Table of Contents. Align the two slots on the bottle holder with the two pegs on the rail. SpectraMax iD5. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. 3 Pages. Products : General, FilterMax F5 reader. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. The amount of colorimetric product was continuously monitored for 120 min. – ™FilterMax F5 Multi-Mode Microplate Reader (cat. Our SpectraTest® ABS2, FL1, LM1, and Multi-Mode Validation Packages provide automated,A delightfully easy to use multimode reader. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. SpectraMax Quant dsDNA Assay Kits. Neurotransmitter Transporter Uptake Assay Kit. MDA is one of the final products of LPO. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. 5% (v/v) to avoid toxicity. Hit enter to search or ESC to close. The hemolysis was calculated with the following formula: Hemolysis (%) = (A sample − A PBS)/(A Triton − A PBS. Activation of TLR4 and TLR2 receptors. , Ltd. 3 Pages. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Category 2 and 3 microplate readers use gradient heating as well. MetaMorph NX. The concentration was expressed as μmol/mg of total protein, based on the use of a standard curve of chloramine T (0-100 μmol/L). Reaction was stopped by the addition of 100 μl Stop Solution and optical density of each well was determined using a FilterMax F5 Multi-Mode microplate reader and SoftMax Pro 6. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). 00FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. LDH cytotoxicity assaySome of new FilterMax F5 Multi-Mode Microplate readers are shipped with ARP USB to serial port adaptor (Manufacturer No: 572213, ARP No: 465338, 1 X USB, 1 X serial DB9/m, blue). ScanLater Western Blot Detection System. ”SpectraMax® Paradigm® Multi-Mode Detection Platform. Mettler Jr. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. Important things to consider when purchasing a multimode microplate reader. FilterMax F5 Multi-Mode Microplate Reader. 6 to 25 ng of prokaryotic recombinant protein reaching a plateau in concentrations above 25 ng. Measurement of Caspase 3/7 Activity. Click Run. 425 or. Affordability with unmatched assay performance in a multi-mode microplate reader The FilterMax™ F3 and F5 Multi-Mode Microplate Readers provide unmatched value and flexibility for a variety of applications capable of performing: - Absorbance detection in the visible range (340 nm to 650 nm, F3) or UV and visible range (230nm to. Absorbance Detection Comparison Table. Ease of use. Recommended Optics • Use the touchscreen to easily set up protocols, run experiments or view tutorial videos • Automatically identify filter sets using NFC functionality, simplifying workflows and eliminating confusion • Perform western blot detection • Push data to workstations with QuickSync, eliminating the need to retrieve data from the instrument SpectraMax iD5 Multi-Mode Microplate Reader Five-mode. 3 Pages. Installation Qualification (IQ)Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). The solution was incubated for 15 min and fluorescence was measured on a SpectraMax microplate reader (Molecular Devices). Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. searchPolymerization and polymerization kinetics were monitored in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA) using a FilterMax F5 Multi-Mode Microplate Reader. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. ABS Test results. 6 reviews. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Devices FilterMax Multi-Mode Microplate Readers. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Page: 1/4 NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. 3 Pages. The Evans blue dye was extracted from the ears with 1 ml of formamide overnight at 55°C and measured spectrophotometrically at 620 nm (FilterMax TM F5 Multi-Mode Microplate Reader). At least three independent experiments were performed for each. Relative firefly and Renilla luciferase units were measured in a FilterMax F5 Multi-Mode Microplate Reader using the Dual-Luciferase ® Reporter Assay System (Promega) according to the manufacturer’s instructions. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® Europium• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. GenePix 4300/4400 Microarray Scanner. Cover plate with gas-permeable membrane and put in incubator. Caspase-3 Activity. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. 3 Pages. The measured temperature can be used to provide feedback. MS-3 Laser Bar Code Reader. SpectraMax® iD5 Multi-Mode Microplate Reader (Molecular Devices cat. After incubation for 2 h at 37 °C, the mixture was centrifuged at 2200 rpm for 10 min to obtain the supernatant. How to Get Multi-Day Kinetic Data. Open SoftMax® Pro software. In the case of cocultures, donor and recipient. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 020 fmol 0. Images were captured using Molecular Devices CloneSelect Imager at a suitable time point post plating. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Activation of TLR4 and TLR2 receptors. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. 4 Pages. time plots at 30, 60, and 90 min using MATLAB ® R2017b (9. , Shanghai, China) and microplate reader (FilterMax F5 Multi-Mode Microplate Reader, LLC, Molecular Devices, San Jose, CA, United States) at a wavelength of 450 nm. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 4 Pages. Ultrafast Multi-Elemental Analysis Atomic Spectroscopy Consumables & Accessories. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. : F5: Accuracy: \ Detection Limit (384-Well): 0. 8 Pages. PHA content was calculated based on the equation (fluorescent intensity/OD 600 × 0. Fluorescent intensity was measured at excitation (485 nm) and emission (595 nm) with the FilterMax F5 Multi-mode microplate reader (Molecular Devices). FilterMax F5 Multi-Mode Microplate Reader. performs within its specifications. Download Table of Contents. It can be upgraded into a powerful multi-mode reader with up to seven detection modes. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Devices FilterMax Multi-Mode Microplate Readers. 2. F3/F5 Multi-Mode Microplate Readers. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia. CloneMatrix. Click “Eject Plate Carrier. please click here. 50/50. (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. MetaMorph NX. You can determine the model and type of your F5 hardware platform through the command line or the Configuration utility. 8. Excitation Filter – EXP1 F535/25, F485/20, F485/20, F370/TRF. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. 4 (Molecular Devices, Sunnyvale, CA, USA) at room temperature (fluorimeter settings used were as follows: measurement type, kinetic, 60 cycle, 60-s interval; excitation wavelength, 360 nm;. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. FilterMax F5 Multi-Mode Microplate Reader. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Page 24: Synergy™ Ht Multi-Mode Microplate Reader Internal plate shaking is also supported. Luminescence was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by extrapolation from a standard curve made in a four-parameter nonlinear regression program in Softmax Pro software (Molecular Devices). Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. By category. Ammonia and NO 2 Ϫ / NO 3 Ϫ analyses were conducted according to established protocols (37, 38), using a FilterMax F5 multimode microplate reader (Molecular Devices, Canada). Instrumentation and Quality Control. The ELISA test was performed using the supernatants from the morphological test. org) with optimized instrument settings b. A 1:1 binding ratio Hill model was used to fit the binding interaction data. Absorbance was measured at 450 nm using a FilterMAx F5 Multi-Mode Microplate Reader and EX2 filter after a 2-hour incubation at 37°C with 10 μL of CCK-8 per 100 μL of media. 2. CatchPoint cGMP Detection Assay Kit. SpectraMax Glo Steady-Luc Reporter Assay Kit. Each condition was performed in. Approximately 50 μl of preculture were inoculated into 1 mL MMAB, leading to a starting density of 0. 00The microplate reader market was valued at US$ 534. 1. CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF. F5 Multi-Mode Microplate Reader. SpectraMax iD3 and iD5 Multi-Mode Microplate Readers;. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. SpectraMax Quant dsDNA Assay Kits. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. 380 or. --> Life sciences. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. 00 USD. 5. Select the protocol “Concentration_pico_green_v3. This document is provided to customers. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. 1. #6590-0118) Filter 665/10 (Molecular Devices cat. Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3. F5 Multi-Mode Microplate Reader. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. FilterMax F3 & F5 Multi-Mode Microplate Readers from. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. com B. 09 CAD. ( C ) H2A. By category. 713579). Delete from my manuals. RNA extraction and reverse transcriptase–quantitative PCR. Synergy HTX laboratory equipment pdf manual download. Sunnyvale, Calif. The data were analyzed using Multi-Mode Analysis software and normalized to fluorescence in the vehicle-treated cells (% of control). Figure 2-23: Bottle holder To install the bottle holder: 1. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. Supernatant (25 μL), or standard, was added and mixed. Laser-aided direct metal deposition of metals and alloys. Molecular Devices Multi-Mode Microplate Reader FilterMax F5. , San Diego, CA, USA). Absorbance detection in the UV and visible range (230 nm to 650 nm) Fluorescence intensity; top read and bottom read. MetaXpress. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. . F3/F5 Multi-Mode Microplate ReadersThe plate was incubated for 1 hr at room temperature with shaking. Supplier: Molecular Devices.